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Dose response of litter weights in rats.

Usage

data("litter")

Format

This data frame contains the following variables

dose

dosages at four levels: 0, 5, 50, 500.

gesttime

gestation time as covariate.

number

number of animals in litter as covariate.

weight

response variable: average post-birth weights in the entire litter.

Details

Pregnant mice were divided into four groups and the compound in four different doses was administered during pregnancy. Their litters were evaluated for birth weights, see multcomp::westfall1999, page 109, and multcomp::westfall1997.

References

*

Examples


  ### fit ANCOVA model to data
  amod <- aov(weight ~ dose + gesttime + number, data = litter)

  ### define matrix of linear hypotheses for `dose'
  doselev <- as.integer(levels(litter$dose))
  K <- rbind(contrMat(table(litter$dose), "Tukey"),
             otrend = c(-1.5, -0.5, 0.5, 1.5),
             atrend = doselev - mean(doselev),
             ltrend = log(1:4) - mean(log(1:4)))

  ### set up multiple comparison object
  Kht <- glht(amod, linfct = mcp(dose = K), alternative = "less")

  ### cf. Westfall (1997, Table 2)
  summary(Kht, test = univariate())
#> 
#> 	 Simultaneous Tests for General Linear Hypotheses
#> 
#> Multiple Comparisons of Means: User-defined Contrasts
#> 
#> 
#> Fit: aov(formula = weight ~ dose + gesttime + number, data = litter)
#> 
#> Linear Hypotheses:
#>                Estimate Std. Error t value  Pr(<t)   
#> 5 - 0 >= 0      -3.3524     1.2908  -2.597 0.00575 **
#> 50 - 0 >= 0     -2.2909     1.3384  -1.712 0.04576 * 
#> 500 - 0 >= 0    -2.6752     1.3343  -2.005 0.02448 * 
#> 50 - 5 >= 0      1.0615     1.3973   0.760 0.77498   
#> 500 - 5 >= 0     0.6772     1.3394   0.506 0.69260   
#> 500 - 50 >= 0   -0.3844     1.4510  -0.265 0.39595   
#> otrend >= 0     -3.4821     2.0867  -1.669 0.04988 * 
#> atrend >= 0   -314.7324   408.9901  -0.770 0.22212   
#> ltrend >= 0     -1.9400     0.9616  -2.018 0.02379 * 
#> ---
#> Signif. codes:  0 ‘***’ 0.001 ‘**’ 0.01 ‘*’ 0.05 ‘.’ 0.1 ‘ ’ 1
#> (Univariate p values reported)
#> 
  summary(Kht, test = adjusted("bonferroni"))
#> 
#> 	 Simultaneous Tests for General Linear Hypotheses
#> 
#> Multiple Comparisons of Means: User-defined Contrasts
#> 
#> 
#> Fit: aov(formula = weight ~ dose + gesttime + number, data = litter)
#> 
#> Linear Hypotheses:
#>                Estimate Std. Error t value Pr(<t)  
#> 5 - 0 >= 0      -3.3524     1.2908  -2.597 0.0518 .
#> 50 - 0 >= 0     -2.2909     1.3384  -1.712 0.4118  
#> 500 - 0 >= 0    -2.6752     1.3343  -2.005 0.2203  
#> 50 - 5 >= 0      1.0615     1.3973   0.760 1.0000  
#> 500 - 5 >= 0     0.6772     1.3394   0.506 1.0000  
#> 500 - 50 >= 0   -0.3844     1.4510  -0.265 1.0000  
#> otrend >= 0     -3.4821     2.0867  -1.669 0.4490  
#> atrend >= 0   -314.7324   408.9901  -0.770 1.0000  
#> ltrend >= 0     -1.9400     0.9616  -2.018 0.2141  
#> ---
#> Signif. codes:  0 ‘***’ 0.001 ‘**’ 0.01 ‘*’ 0.05 ‘.’ 0.1 ‘ ’ 1
#> (Adjusted p values reported -- bonferroni method)
#> 
  summary(Kht, test = adjusted("Shaffer"))
#> 
#> 	 Simultaneous Tests for General Linear Hypotheses
#> 
#> Multiple Comparisons of Means: User-defined Contrasts
#> 
#> 
#> Fit: aov(formula = weight ~ dose + gesttime + number, data = litter)
#> 
#> Linear Hypotheses:
#>                Estimate Std. Error t value Pr(<t)  
#> 5 - 0 >= 0      -3.3524     1.2908  -2.597 0.0518 .
#> 50 - 0 >= 0     -2.2909     1.3384  -1.712 0.0915 .
#> 500 - 0 >= 0    -2.6752     1.3343  -2.005 0.0734 .
#> 50 - 5 >= 0      1.0615     1.3973   0.760 1.0000  
#> 500 - 5 >= 0     0.6772     1.3394   0.506 1.0000  
#> 500 - 50 >= 0   -0.3844     1.4510  -0.265 1.0000  
#> otrend >= 0     -3.4821     2.0867  -1.669 0.0998 .
#> atrend >= 0   -314.7324   408.9901  -0.770 0.4442  
#> ltrend >= 0     -1.9400     0.9616  -2.018 0.0518 .
#> ---
#> Signif. codes:  0 ‘***’ 0.001 ‘**’ 0.01 ‘*’ 0.05 ‘.’ 0.1 ‘ ’ 1
#> (Adjusted p values reported -- Shaffer method)
#> 
  summary(Kht, test = adjusted("Westfall"))
#> 
#> 	 Simultaneous Tests for General Linear Hypotheses
#> 
#> Multiple Comparisons of Means: User-defined Contrasts
#> 
#> 
#> Fit: aov(formula = weight ~ dose + gesttime + number, data = litter)
#> 
#> Linear Hypotheses:
#>                Estimate Std. Error t value Pr(<t)  
#> 5 - 0 >= 0      -3.3524     1.2908  -2.597 0.0319 *
#> 50 - 0 >= 0     -2.2909     1.3384  -1.712 0.0893 .
#> 500 - 0 >= 0    -2.6752     1.3343  -2.005 0.0646 .
#> 50 - 5 >= 0      1.0615     1.3973   0.760 0.7750  
#> 500 - 5 >= 0     0.6772     1.3394   0.506 0.7271  
#> 500 - 50 >= 0   -0.3844     1.4510  -0.265 0.7271  
#> otrend >= 0     -3.4821     2.0867  -1.669 0.0917 .
#> atrend >= 0   -314.7324   408.9901  -0.770 0.3951  
#> ltrend >= 0     -1.9400     0.9616  -2.018 0.0459 *
#> ---
#> Signif. codes:  0 ‘***’ 0.001 ‘**’ 0.01 ‘*’ 0.05 ‘.’ 0.1 ‘ ’ 1
#> (Adjusted p values reported -- Westfall method)
#> 
  summary(Kht, test = adjusted("single-step"))
#> Warning: Completion with error > abseps
#> Warning: Completion with error > abseps
#> 
#> 	 Simultaneous Tests for General Linear Hypotheses
#> 
#> Multiple Comparisons of Means: User-defined Contrasts
#> 
#> 
#> Fit: aov(formula = weight ~ dose + gesttime + number, data = litter)
#> 
#> Linear Hypotheses:
#>                Estimate Std. Error t value Pr(<t)  
#> 5 - 0 >= 0      -3.3524     1.2908  -2.597 0.0322 *
#> 50 - 0 >= 0     -2.2909     1.3384  -1.712 0.2034  
#> 500 - 0 >= 0    -2.6752     1.3343  -2.005 0.1191  
#> 50 - 5 >= 0      1.0615     1.3973   0.760 0.9999  
#> 500 - 5 >= 0     0.6772     1.3394   0.506 0.9987  
#> 500 - 50 >= 0   -0.3844     1.4510  -0.265 0.8907  
#> otrend >= 0     -3.4821     2.0867  -1.669 0.2183  
#> atrend >= 0   -314.7324   408.9901  -0.770 0.6620  
#> ltrend >= 0     -1.9400     0.9616  -2.018 0.1162  
#> ---
#> Signif. codes:  0 ‘***’ 0.001 ‘**’ 0.01 ‘*’ 0.05 ‘.’ 0.1 ‘ ’ 1
#> (Adjusted p values reported -- single-step method)
#>